rabbit anti notch4  (Santa Cruz Biotechnology)

Journal: International Journal of Oncology

Article Title: Regulation of gliomagenesis and stemness through acid sensor ASIC1a

doi: 10.3892/ijo.2021.5262

Figure Lengend Snippet: Notch signaling is negatively associated with ASIC1a in glioblastoma multiforme. (A-C) The glioma cells were transfected with shASIC1a and control followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4, and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting in (A) A172, (B) U87MG, and (C) PDX-L12 cells. (D-F) Subsequently, the glioma cells (D) A172, (E) U87MG and (F) PDX-L12 were transfected with ASIC1a-FLAG followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4 and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting. * P<0.05 and ** P<0.01. ASIC1a, acid-sensing ion channel 1a; sh, short hairpin; GSC, glioblastoma stem cells; ALDH1, aldehyde dehydrogenase 1.

Article Snippet: The following primary antibodies were used in the present study: Rabbit monoclonal anti-ASIC1a antibody (cat. no. 35-156465) was purchased from American Research Products, Inc. Rabbit polyclonal anti-Notch4 antibody (cat. no. 07-189) and anti-β-actin antibody (product no. A3854) were purchased from Sigma-Aldrich; Merck KGaA.

Techniques: Transfection, Control, Expressing, Western Blot

Journal: Acta Histochemica et Cytochemica

Article Title: Notch Signaling May Be Involved in the Abnormal Differentiation of Epidermal Keratinocytes in Psoriasis

doi: 10.1267/ahc.14027

Figure Lengend Snippet: Immunohistochemical localization of Notch1 ( A, B ), Notch2 ( C, D ), Notch3 ( E, F ), and Notch4 ( G, H ). Representative examples from normal skin ( A, C, E, G ) and psoriatic skin ( B, D, F, H ). (Original magnification ×100). In psoriatic skin, Notch1, 2, and 4 localized in suprabasal layers and Notch3 localized in pan epidermal. Black arrows indicate positive cells.

Article Snippet: Rabbit anti-human Notch3 antibody (Santa Cruz Biotechnology) was used at a 1:50 dilution, and rabbit anti-human Notch4 antibody (Santa Cruz Biotechnology) was used at a 1:100 dilution.

Techniques: Immunohistochemical staining

Journal: Acta Histochemica et Cytochemica

Article Title: Notch Signaling May Be Involved in the Abnormal Differentiation of Epidermal Keratinocytes in Psoriasis

doi: 10.1267/ahc.14027

Figure Lengend Snippet: Intensity of immunostaining for Notch1, 2, 3, and 4 in normal and psoriatic epidermis

Article Snippet: Rabbit anti-human Notch3 antibody (Santa Cruz Biotechnology) was used at a 1:50 dilution, and rabbit anti-human Notch4 antibody (Santa Cruz Biotechnology) was used at a 1:100 dilution.

Techniques: Immunostaining

Journal: Acta Histochemica et Cytochemica

Article Title: Notch Signaling May Be Involved in the Abnormal Differentiation of Epidermal Keratinocytes in Psoriasis

doi: 10.1267/ahc.14027

Figure Lengend Snippet: Quantitative real-time RT-PCR analysis of Notch mRNA in the normal and psoriatic epidermis using laser microdissection. A , Notch1; B , ‍Notch2; C , Notch3; D , Notch4. mRNA expressions were analyzed by quantitative RT-PCR. *P<0.05.

Article Snippet: Rabbit anti-human Notch3 antibody (Santa Cruz Biotechnology) was used at a 1:50 dilution, and rabbit anti-human Notch4 antibody (Santa Cruz Biotechnology) was used at a 1:100 dilution.

Techniques: Quantitative RT-PCR, Laser Capture Microdissection

Journal: Acta Histochemica et Cytochemica

Article Title: Notch Signaling May Be Involved in the Abnormal Differentiation of Epidermal Keratinocytes in Psoriasis

doi: 10.1267/ahc.14027

Figure Lengend Snippet: Localization of Notch1, 2, 3, and 4 in normal and psoriatic epidermis

Article Snippet: Rabbit anti-human Notch3 antibody (Santa Cruz Biotechnology) was used at a 1:50 dilution, and rabbit anti-human Notch4 antibody (Santa Cruz Biotechnology) was used at a 1:100 dilution.

Techniques:

Journal: BMC Genomics

Article Title: Characterization of gene expression profiles for different types of mast cells pooled from mouse stomach subregions by an RNA amplification method

doi: 10.1186/1471-2164-10-35

Figure Lengend Snippet: Summary of genes examined by real-time PCR analysis.

Article Snippet: For tissue staining, frozen sections were fixed in 4% formaldehyde and incubated with a rabbit anti-Notch4 antibody (1:20, Santa Cruz Biotechnology, Santa Cruz, CA) or a rabbit anti-Ptgr1 antibody (1:20) which was a kind gift from Prof. Takao Shimizu (University of Tokyo) [ ].

Techniques: Real-time Polymerase Chain Reaction

Journal: BMC Genomics

Article Title: Characterization of gene expression profiles for different types of mast cells pooled from mouse stomach subregions by an RNA amplification method

doi: 10.1186/1471-2164-10-35

Figure Lengend Snippet: Immunohistochemical analysis of Notch4 and Ptgr1 in sMCs and mMCs in stomach tissue . ( a ) Stomach submucosa (sMCs; left panels ), stomach mucosa (mMCs; middle panels ) and skin (skin MCs; right panels ) sections were stained with an anti-Notch4 antibody ( lower panels ) and with toluidine blue ( upper panels ). sMCs stained with the anti-Notch4 antibody in the gastric submucosa and skin dermis are indicated by arrows. No staining was observed in mMCs ( arrowheads ) localized in the gastric mucosa. sMCs and mMCs were metachromatically stained with toluidine blue. ( b ) Stomach submucosa (sMCs; left panels ) and stomach mucosa (mMCs; right panels ) sections were stained with an anti-Ptgr1 antibody ( lower panels ) and with toluidine blue ( upper panels ). No staining with the anti-Ptgr1 antibody was found in the sMCs ( arrow ). Small signals were observed in the mMCs ( arrowheads ). sMCs and mMCs were metachromatically stained with toluidine blue. Bars , 25 μm (a, b).

Article Snippet: For tissue staining, frozen sections were fixed in 4% formaldehyde and incubated with a rabbit anti-Notch4 antibody (1:20, Santa Cruz Biotechnology, Santa Cruz, CA) or a rabbit anti-Ptgr1 antibody (1:20) which was a kind gift from Prof. Takao Shimizu (University of Tokyo) [ ].

Techniques: Immunohistochemical staining, Staining

Journal: BMC Genomics

Article Title: Characterization of gene expression profiles for different types of mast cells pooled from mouse stomach subregions by an RNA amplification method

doi: 10.1186/1471-2164-10-35

Figure Lengend Snippet: List of primers used for real-time PCR analysis.

Article Snippet: For tissue staining, frozen sections were fixed in 4% formaldehyde and incubated with a rabbit anti-Notch4 antibody (1:20, Santa Cruz Biotechnology, Santa Cruz, CA) or a rabbit anti-Ptgr1 antibody (1:20) which was a kind gift from Prof. Takao Shimizu (University of Tokyo) [ ].

Techniques: Real-time Polymerase Chain Reaction